Treatment of human metaphase chromosomes with 5 mM reducing reagents Biology Diagrams
Treatment of human metaphase chromosomes with 5 mM reducing reagents Biology Diagrams Chromosome misalignments cause spindle misorientation. To study the role of Spindly in spindle positioning, we filmed cells grown on rectangular‐shaped micropatterns and measured the angle of the metaphase plate relative to the short cell axis (Fig (Fig1A). 1 A). Since Spindly depletion leads to chromosome alignment defects associated with a profound delay in mitosis (20 and Fig EV1 A), we
The kinetochore, the protein complex assembled at the centromere of each mitotic chromosome, serves as the attachment site for the spindle MTs. A combination of forces generated by kinetochores and microtubule dynamics is thought to contribute to kinetochore-MT attachment and chromosome movement in achieving metaphase chromosome alignment.
New Insights into the Mechanism for Chromosome Alignment in Metaphase Biology Diagrams
Understanding these phases highlights the mechanisms ensuring accurate chromosome segregation. Errors in these steps can lead to genetic abnormalities, making their regulation crucial for maintaining cellular integrity. Metaphase: Central Alignment. Metaphase is a pivotal stage where chromosomes align in preparation for segregation. Intriguingly, FISH with chromosome-specific probes showed that, despite the defects resulting from KIF18A inactivation, there were no detectable alterations in chromosome copy number . Overall, these data suggest that although chromosome alignment prevents the formation of lagging chromosomes and micronuclei in anaphase, mechanisms exist that
PAK2 is essential for chromosome alignment in metaphase I oocytes. The specific localization of PAK2 during oocyte maturation prompted us to ask whether Pak2 functions in the chromosome alignment
Chromosome Alignment and Segregation Regulated by ... Biology Diagrams
(We defined misaligned chromosomes in metaphase as chromosomes that failed to align with the majority of chromosomes at metaphase plates.) (SD) from at least three independent experiments. For chromosome segregation defects, 100 mitotic cells were analyzed for each RNAi experiment. Scale bars in (C) and (D), 20 μm.
Defects in chromosome alignment are normally avoided by increased Aurora B activity at centromeres of misaligned chromosomes. 27 However, the correction of erroneous attachments underlying some chromosome alignment defects (e.g., syntelic attachments) appears to be less robust in cancer cells that also show overly stabilized kinetochore
